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Patent US10364416
Issued 2019-07-30
Cannabichromenic Acid Synthase From Cannabis Sativa
Nucleic acid molecules from cannabis have been isolated and characterized and encode polypeptides having cannabichromenic acid synthase activity. Expression or over-expression of the nucleic acids alters levels of cannabinoid compounds. The polypeptides may be used in vivo or in vitro to produce cannabinoid compounds.
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- 1. A method of modulating levels of cannabinoid compounds in an organism, cell or tissue, said method comprising:
a) introducing a nucleic acid molecule comprising:
i) a nucleotide sequence complementary to a polynucleotide encoding at least a portion of a polypeptide having the sequence set for in SEQ ID NO: 2 or 6 or a polypeptide having at least 99.5% sequence identity to SEQ ID NO: 2 or 6; or
ii) a nucleotide sequence complementary to a polynucleotide having at least, greater than or about 99.5% sequence identity to at least a portion of SEQ ID NO: 1 or 5;
into the organism, cell or tissue to decrease the level of a cannabinoid compound,
wherein the portion comprises greater than, at least or about 15 contiguous nucleotides,
and wherein the nucleic acid molecule is comprised in a construct, to silence cannabichromenic acid synthase gene in the organism, cell or tissue to decrease the level of a cannabinoid compound; or
b) introducing a nucleic acid molecule or construct comprising said nucleic acid molecule, and expressing or over-expressing a polypeptide having cannabichromenic acid synthase activity encoded by said nucleic acid molecule, said nucleic acid molecule comprising
i) a nucleotide sequence encoding a polypeptide having the sequence set for in SEQ ID NO: 2 or 6 or a polypeptide having at least 99.5% sequence identity to SEQ ID NO: 2 or 6;
ii) a nucleotide sequence having at least, greater than or about 99.5% sequence identity to SEQ ID NO: 1 or 5; or
iii) a nucleotide sequence having at least, greater than or about 90% sequence identity to SEQ ID NO: 8 or 9;
in the organism, cell or tissue to increase the level of a cannabinoid compound.
