The present invention is a method for the biosynthesis of hundreds of compounds, mainly found in the cannabis plant. The starting material for these compounds can be any biological compound that is used/produced in a biological organism from the sugar family starting materials or other low cost raw materials processed via enzymes or within organisms to give final products. These final products include, but are not limited to: cannabinoids, terpenoids, stilbenoids, flavonoids, phenolic amides, lignanamides, spermidine alkaloids, and phenylpropanoids. Specifically, the present invention relates to the regular, modified, or synthetic gene(s) of select enzymes that are processed and inserted into an expression system (for example, a vector, cosmid, BAC, YAC, phage) to produce modified hosts. The modified host is then optimized for efficient production and yield via manipulation, silencing, and amplifying inserted or other genes in the host, leading to an efficient system for product.
Claim CLM-00001. 1. An isolated nucleic acid molecule comprising a nucleotide sequence that is at least 95% identical to the nucleotides 5052 to 6671 of SEQ ID NO: 35, wherein the isolated nucleic acid molecule: (i) is non-naturally occurring; (ii) has a nucleotide sequence that is codon-optimized for expression in a host organism and encodes olivetolic acid cyclase (OAC) polypeptide; and (iii) produces olivetolic acid or divarinolic acid in the host organism when inserted and expressed in the host organism, and wherein the host organism is Escherichia coli, Saccharomyces cerevisiae or Pichia pastoris.
Claim CLM-00002. 2. A host organism that comprises an isolated nucleic acid molecule comprising a nucleotide sequence that is at least 95% identical to the nucleotides 5052 to 6671 of SEQ ID NO: 35, wherein the isolated nucleic acid molecule: (i) is non-naturally occurring; (ii) has a nucleotide sequence that is codon-optimized for expression in the host organism and encodes olivetolic acid cyclase (OAC) polypeptide; and (iii) produces olivetolic acid or divarinolic acid in the host organism when inserted and expressed in the host organism, and wherein the host organism is a bacterium, plant alga or fungus.
