Patent US10627397

Multisignal reagents for labeling analytes
[+] Show Details

US10627397

1. A competitive binding assay composition comprising: (5) (4)

(a) a sample suspected of containing an analyte;

(b) a detection reagent comprising the analyte or an analog thereof bound covalently to a first nucleic acid strand, and

a second nucleic acid strand complementary with and hybridized to the first nucleic acid strand, wherein the second nucleic acid strand comprises more than one first member of a binding pair of which the analyte is not a member, and

wherein the binding pair is a sugar-lectin, an antigen-antibody, a ligand-ligand receptor, a hormone-hormone receptor, an enzyme-substrate, a biotin-avidin, or a biotin-streptavidin binding pair; and

(c) a binding agent capable of binding to the analyte or the analog thereof of the detection reagent and to analyte contained in the sample,

wherein the analyte is cAMP, cGMP, 8-bromoadenosine 3′,5′-cyclic monophosphate, cholesterol, a hydroxycholesterol, vitamin D, 25-hydroxy vitamin D, vitamin B12, vitamin E, vitamin B1, vitamin B6, ascorbic acid, retinol, biotin, folate, legumin, salbutamol, melamine, sulfaquinoxaline, an inositol phosphate, a phosphatidyl inositol phosphate, prednisone, pregnenolone, dexamethasone, triamcinolone, fludrocortisone, dihydrotachysterol, oxandrolone, testosterone, dihydrotestosterone, nandrolone, norethindrone, medroxyprogesterone acetate, progesterone, a glucocorticoid, aldosterone, estrogen, oxytocin, androstanediol glucuronide, bilirubin, warfarin, an estrogen, methotrexate, tobramycin, acetaminophen, encainide, fluoxetine, gentamycin, an aminoglycoside, amikacin, coenzyme Q10, theophylline, phenytoin, cimetidine, disulfiram, trazodone, ethanol, halothane, phenylbutazone, azapropazone, ibuprofen, amiodarone, imipramine, miconazole, metronidazole, nifedipine, chloramphenicol, trimethoprim, a sulfonamide, rifampin, cisplatin, vinblastine, bleomycin, oxacillin, nitrofurantoin, phenobarbital, primidone, carbamazepine, metoclopramide, cholestyramine, colestipol, neomycin, sulfasalazine, digoxin, indomethacin, diltiazem, erythromycin, tetracycline, itraconazole, nicardipine, triamterene, spironolactone, chlorpromazine, a cyclosporin, nortriptyline, ethosuximide, imipramine, codeine, lorazepam, topiramate, disopyramide, levetiracetam, clobazam, oxcarbazepine, fructosamine, caffeine, methaqualone, meprobamate, fluphenazine, a barbiturate, a phenothiazine, serotonin, valproic acid, digitoxin, maprotiline, lidocaine, mexiletine, primidone, risperidone, [OH-risperidone] paliperidone, a porphyrin, haloperidol, flecainide, tocainide, acetazolamide, a sulfonamide, verapamil, a metanephrine, an oxidized nucleotide, a mycotoxin, tetrahydrocannabinol, a cannabinoid, cocaine, LSD, an amphetamine, heroin, methadone, nicotine, cotinine, a benzodiazepine, bis(2-ethylhexyl) phthalate, bisphenol A, hydralazine, atrazine, an organochloride insecticide, an organophosphate insecticide, or a carbamate insecticide.

2. The composition of claim 1, wherein the analyte is oxytocin, cAMP, cGMP, hydroxycholesterol, vitamin D, or 25-hydroxy vitamin D. (1) (0)

4. The composition of claim 1, wherein the binding agent is an antibody or an analyte-binding fragment thereof. (0)

5. The composition of claim 1, wherein the binding agent is bound to a solid phase. (1) (0)

7. The composition of claim 1, further comprising second members of the binding pair bound to the first members of the binding pair. (1) (0)

10. The composition of claim 1, wherein the detection reagent (b) consists essentially of: (3) (2)

the analyte or an analog thereof bound covalently to the first nucleic acid strand, and

the second nucleic acid strand complementary with and hybridized to the first nucleic acid strand, wherein the second nucleic acid strand comprises more than one first member of a binding pair of which the analyte is not a member, and

wherein the binding pair is a sugar-lectin, an antigen-antibody, a ligand-ligand receptor, a hormone-hormone receptor, an enzyme-substrate, a biotin-avidin, or a biotin-streptavidin binding pair.

11. The composition of claim 10, wherein the analyte is oxytocin, cAMP, cGMP, hydroxycholesterol, vitamin D, or 25-hydroxy vitamin D. (1) (0)

13. The composition of claim 10, wherein the binding agent is an antibody or an analyte-binding fragment thereof. (0)

14. The composition of claim 10, wherein the binding agent is bound to a solid phase. (1) (0)

View Abstract and Specification Size

PDF with Images and Document Face >

Full Text Publication >

Patent US10627397

Multisignal reagents for labeling analytes

Patent Matrix® Search