| Abstract: |
In an aspect, the disclosure provides methods for making neurotransmitters in a host organism. The neurotransmitters can be cannabinoids and derivatives of cannabinoids. The host cells can be microalgae, fungi or other host cells. In a related aspect, the disclosure provides host cells engineered to have biochemical pathways for making neurotransmitters such as cannabinoids. |
| Inventor: |
Rudenko, George (Mountain View, CA, US); Evans, Robert (Danville, CA, US) |
| Applicant: |
Purissima, Inc. (South San Francisco, CA, US) |
| Face Assignee: |
Purissima, Inc. (South San Francisco, CA, US) |
| Filed: |
2018-05-04 |
| Issued: |
2020-07-07 |
| Claims: |
20 |
|
US10704066
|
1. A method, comprising the steps of: providing a Prototheca microalgae host cell wherein the Prototheca microalgae host cell comprises a first nucleic acid encoding a hexanoyl-CoA synthase from Cannabis sativa, a second nucleic acid encoding a 3,5,7-trioxododecanoyl-CoA synthase from Cannabis sativa, a third nucleic acid encoding an olivetolic acid cyclase from Cannabis sativa, and a fourth nucleic acid encoding a geranyl-diphosphate:olivetolate geranyltransferase from Cannabis sativa, wherein the first nucleic acid is operably linked to a control region, wherein the second nucleic acid is operably linked to a control region, wherein the third nucleic acid is operably linked to a control region, and wherein the fourth nucleic acid is operably linked to a control region, introducing the microalgae cell into a media wherein the media comprises a carbon source, culturing the microalgae cell in the media whereby a cannabinoid is made.
(15)
(0)
|
|
