Patent US10724048

Systems and methods for enhancing trichome formation and density in cannabis
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US10724048

1. A method of increasing trichome formation in a plant comprising the steps: (1) (2)

stably transforming a cannabinoid producing plant to express a heterologous polynucleotide sequence according to SEQ ID NO. 7, wherein expression of the heterologous polynucleotide sequence independently upregulates trichome formation and metabolic flux through the cannabinoid biosynthetic pathway in said cannabinoid producing plant; and

wherein said cannabinoid producing plant is Cannabis.

2. The method of claim 1 wherein the Cannabis plant is Cannabis sativa or hemp. (1) (0)

3. The method of claim 2 wherein the nucleotide sequence SEQ ID NO. 7 is operably linked to a promoter to produce an expression vector and wherein said expression vector is configured to be introduced via transformation of a Cannabis sativa or hemp plant. (2) (0)

4. The method of claim 3 wherein the Cannabis sativa or hemp plant is stably transformed through Agrobacterium Ti-plasmid mediated transformation. (1) (0)

6. A genetically modified Cannabis sativa or hemp plant or part thereof produced by the method of claim 3. (2) (0)

7. The genetically modified Cannabis sativa or hemp plant or part thereof and its progeny of claim 6 wherein the plant produces 30% more trichome structures compared to a wild-type Cannabis sativa or hemp plant. (1) (0)

9. The genetically modified Cannabis sativa or hemp plant or part thereof of claim 6 wherein said heterologous polynucleotide sequence according to SEQ ID NO. 7 encodes a heterologous polypeptide according to the amino acid sequence SEQ ID NO. 6 wherein expression of the polynucleotide independently upregulates trichome formation and metabolic flux through the cannabinoid biosynthetic pathway. (1) (0)

11. A method of increasing cannabinoid formation in a plant comprising the steps: (1) (2)

stably transforming a cannabinoid producing plant to express a heterologous polynucleotide sequence according to SEQ ID NO. 7, wherein expression of the heterologous polynucleotide sequence independently upregulates metabolic flux through the cannabinoid biosynthetic pathway increasing formation of CBDA and/or THCA in said cannabinoid producing plant and independently upregulates trichome formation in said cannabinoid producing plant; and

wherein said cannabinoid producing plant is Cannabis.

12. The method of claim 11 wherein the Cannabis plant is Cannabis sativa or hemp. (2) (0)

13. The method of claim 12 wherein the nucleotide sequence SEQ ID NO. 7 is operably linked to a promoter to produce an expression vector and wherein said expression vector is configured to be introduced via transformation to a Cannabis sativa or hemp plant. (1) (0)

15. A genetically modified Cannabis sativa or hemp plant or part thereof produced by the method of claim 12. (3) (0)

16. The genetically modified Cannabis sativa or hemp plant or part thereof and its progeny of claim 15 wherein said plant or parts thereof and its progeny express the amino sequence according to SEQ ID NO. 6 wherein expression of the polynucleotide independently upregulates formation of CBDA and/or THCA and independently upregulates trichome formation. (1) (0)

18. The genetically modified Cannabis sativa or hemp plant or part thereof of claim 15 wherein its progeny is a seed from the genetically modified Cannabis sativa or hemp plant. (0)

19. The genetically modified Cannabis sativa or hemp plant or part thereof and its progeny of claim 15 wherein said nucleotide sequence according to SEQ ID NO. 7 that has been codon optimized for expression in Cannabis sativa or hemp. (0)

20. A method of increasing trichome formation in a plant comprising the steps: (1) (2)

stably transforming a cannabinoid producing plant to express a heterologous polynucleotide sequence encoding transcription factor MYB1 from Artemisia annua, wherein expression of the heterologous MYB1 independently upregulates trichome formation and metabolic flux through the cannabinoid biosynthetic pathway in said cannabinoid producing plant; and

wherein said cannabinoid producing plant is Cannabis.

21. The method of claim 20, wherein said heterologous polynucleotide sequence encoding transcription factor MYB1 from Artemisia annua comprises a heterologous polynucleotide according to SEQ ID NO. 7 that has been codon optimized for expression in Cannabis sativa or hemp. (1) (0)

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Patent US10724048

Systems and methods for enhancing trichome formation and density in cannabis

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