Abstract: |
This disclosure provides methods for determining relative abundance of one or more non-host species in a sample from a host. Also provided are methods involving addition of known concentrations of synthetic nucleic acids to a sample and performing sequencing assays to identify non-host species such as pathogens. Also provided are methods of tracking samples, tracking reagents, and tracking diversity loss in sequencing assays. |
Inventor: |
Christians, Fred C. (Los Altos Hills, CA, US); Vilfan, Igor D. (East Palo Alto, CA, US); Kertesz, Michael (Menlo Park, CA, US); Blauwkamp, Timothy A. (Palo Alto, CA, US); Venkatasubrahmanyam, Shivkumar (San Jose, CA, US); Rosen, Michael (Palo Alto, CA, US); Sit, Rene (Sunnyvale, CA, US) |
Applicant: |
Karius, Inc. (Menlo Park, CA, US) |
Face Assignee: |
KARIUS, INC. (Redwood City, CA, US) |
Filed: |
2017-03-24 |
Issued: |
2018-05-22 |
Claims: |
30 |
|
US9976181
|
1. A method for determining abundance of nucleic acids in an initial sample comprising target nucleic acids, the method comprising:
(19)
(3)
|
|